Orchestrating the cell cycle in yeast: sequential localization of key mitotic regulators at the spindle pole and the bud neck.
نویسندگان
چکیده
1 Departamento de Microbiologı!a II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain 2 Departamento de Microbiologı!a y Gene! tica, Instituto de Microbiologı!a-Bioquı!mica, Edificio Departamental, Campus Miguel de Unamuno, Universidad de Salamanca/CSIC, 37007 Salamanca, Spain 3 Department of Molecular and Cell Biology, Division of Biochemistry and Molecular Biology, University of California, Berkeley, CA 94720, USA
منابع مشابه
Disappearance of the budding yeast Bub2–Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit
Budding yeast spindle position checkpoint is engaged by misoriented spindles and prevents mitotic exit by inhibiting the G protein Tem1 through the GTPase-activating protein (GAP) Bub2/Bfa1. Bub2 and Bfa1 are found on both duplicated spindle pole bodies until anaphase onset, when they disappear from the mother-bound spindle pole under unperturbed conditions. In contrast, when spindles are misor...
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Cdc15p is an essential protein kinase and functions with a group of late mitotic proteins that includes Lte1p, Tem1p, Cdc14p and Dbf2p/Dbf20p to inactivate Cdc28p-Clb2p at the end of mitosis in budding yeast [1] [2]. Cdc14p is activated and released from the nucleolus at late anaphase/telophase to dephosphorylate important regulators of Cdc28p-Clb2p such as Hct1p/Cdh1p, Sic1p and Swi5p in a CDC...
متن کاملStable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.
Faithful partitioning of genetic material during cell division requires accurate spatial and temporal positioning of nuclei within dividing cells. In Saccharomyces cerevisiae, nuclear positioning is regulated by an elegant interplay between components of the actin and microtubule cytoskeletons. Regulators of this process include Bud6p (also referred to as the actin-interacting protein Aip3p) an...
متن کاملThe Surveillance Mechanism of the Spindle Position Checkpoint in Yeast
The spindle position checkpoint in Saccharomyces cerevisiae delays mitotic exit until the spindle has moved into the mother-bud neck, ensuring that each daughter cell inherits a nucleus. The small G protein Tem1p is critical in promoting mitotic exit and is concentrated at the spindle pole destined for the bud. The presumed nucleotide exchange factor for Tem1p, Lte1p, is concentrated in the bud...
متن کاملRegulation of the localization of Dbf2 and mob1 during cell division of saccharomyces cerevisiae.
The mitotic exit network (MEN) governs Cdk inactivation. In budding yeast, MEN consists of the protein phosphatase Cdc14, the ras-like GTPase Tem1, protein kinases Cdc15, Cdc5, Dbf2 and Dbf2-binding protein Mob1. Tem1, Dbf2, Cdc5 and Cdc15 have been reported to be localized at the spindle pole body (SPB). Here we report changes of the localization of Dbf2 and Mob1 during cell division. Dbf2 and...
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ورودعنوان ژورنال:
- Microbiology
دوره 148 Pt 9 شماره
صفحات -
تاریخ انتشار 2002